The study looked at how different levels of nitrogen and biofertilizers affect the growth and yield of barley. They tested three levels of nitrogen and four types of biofertilizers on barley plants. The results showed that using 80 kg/ha of nitrogen along with both Azotobacter and Azospirillum biofertilizers led to the best growth and highest grain yield in barley. Azospirillum and Azotobacter biofertilizers also had positive effects on plant growth and yield compared to not using any biofertilizers.