Biochemistry, Genetics and Molecular Biology
8 years ago

Insoluble protein-dye complexes challenge accuracy of protein assays.

Analytical Biochemistry
Coomassie blue protein dye-binding assays measure formation of an insoluble protein-dye complex
T. C. Marshall, Katherine M. Williams

Paper Summary

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The researchers found that the protein-Coomassie brilliant blue complexes formed in protein dye-binding assays are insoluble. This was determined by observing complete loss of color in the supernates and filtrates of the assay mix. The solubility of proteins in the dye reagent affects how the assay responds to different proteins and their macromolecular structures.