Medicine
8 years ago

New RT-PCR test detects SARS and other coronaviruses with high sensitivity.

Journal of Virological Methods
Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
Dena Adachi, Grant Johnson, Ryan Draker, Melissa Ayers, Tony Mazzulli, Pierre J. Talbot, Raymond Tellier

Paper Summary

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A single RT-PCR test can detect the SARS-associated coronavirus and other human coronaviruses with high sensitivity. The test can identify the specific virus by sequencing the results. During the SARS outbreak in Toronto, a quick screening test using restriction enzyme analysis was found to be very helpful.