Biochemistry, Genetics and Molecular Biology
8 years ago

Spinach chloroplasts reveal key to sustainable energy production.

Biochimica et Biophysica Acta (BBA) - Bioenergetics
Further characterization of a Photosystem-II particle isolated from spinach chloroplasts by triton treatment: The reaction-center components
Bacon Ke, Saura C. Sahu, Elwood R. Shaw, Helmut Beinert

Paper Summary

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The researchers studied a Photosystem-II particle from spinach chloroplasts using Triton treatment. They found that the particle has a chlorophyll ab ratio of 28±2 and is rich in manganese. The particle contains C550 and P680, which are key components of Photosystem II. The presence of P680 was confirmed by low-temperature EPR spectroscopy. The particle does not have P700 or iron-sulfur proteins. Photobleaching of C550 and P680 at 77 °K is irreversible. The particle is enriched in P680, C550, and cytochrome b559 compared to unfractionated chloroplasts.