The research looked at how the time between removing the canine epididymis and cooling it affects the quality of sperm after thawing. Sperm from the epididymis stored at 4°C or 20°C for 6 hours showed similar motility. But storing it at 20°C for 12 hours or longer decreased sperm motility compared to 4°C. Sperm quality after thawing from the caudal epididymides stored at 20°C for 0 or 6 hours and then at 4°C for 24 hours was similar. Thus, keeping the canine epididymis at 20°C for up to 6 hours after removal may not greatly affect the quality of thawed caudal epididymal sperm.