The researchers looked at how different treatments affect the ability of boar sperm to fertilize eggs in a lab setting. They used sperm from the epididymis (a part of the male reproductive system) and from ejaculate, and treated them in three ways: unwashed, washed with a solution containing BSA, and washed on a Percoll gradient. They found that epididymal sperm had better motility and fertilization ability compared to ejaculated sperm. The Percoll-treated sperm showed the highest motility and fertilization ability, but also had some drawbacks. Overall, the findings suggest that certain treatments can help ejaculated sperm reach the same fertilization levels as epididymal sperm.