Thyroid hormones and glucocorticoids work together to control low affinity glucocorticoid binding sites in the liver of male rats. When thyroid hormones were reduced in rats, the binding sites decreased by 90%, but giving them T3 brought the levels back up. Removing the adrenal glands caused a 50% drop in binding sites, which could be fixed by giving corticosterone acetate. Rats without thyroid hormones and adrenal glands had very low binding sites, but giving them both corticosterone acetate and T3 brought the levels back up. This shows that both thyroid hormones and glucocorticoids are important for controlling these binding sites, and they work together to do so.