Discovery of Key Enzymes Essential for Starch Granule Biosynthesis
Debranching enzymes play a role in making starch granules in plants. When all debranching enzymes are removed in Arabidopsis, starch granules are not formed, and instead, highly branched glucans are produced. These glucans are different from normal starch and form small, insoluble particles. However, these glucans are broken down by other enzymes, leading to the production of maltose and branched malto-oligosaccharides. Removing a specific alpha-amylase enzyme can restore the production of starch granules in the absence of debranching enzymes. This suggests that debranching enzymes are not essential for making starch granules, but they help in the process of starch crystallization.