The study looked at how Datura stramonium L. leaves process nitrogen from nitrate. They used a special technique with 15N and a chemical inhibitor to see which pathways were involved. Without the inhibitor, glutamate had the most 15N, and glutamine accumulated the most 15N at high nitrate levels. With the inhibitor, most amino compounds lost 15N, except for glutamine and threonine. This suggests that the glutamine synthetase-GOGAT pathway is crucial for turning nitrate into amino acids in Datura leaves.