Arsenic exposure can harm male reproductive health by affecting sperm production. In this study, mice were given water with different levels of arsenic for 60 days. The mice exposed to arsenic had smaller testes and epididymis, lower sperm quality, and abnormal sperm. The gene Ddx3y, important for sperm development, was found to be significantly reduced in the testes and epididymis of arsenic-exposed mice. This suggests that arsenic can disrupt sperm production and may target the Ddx3y gene, leading to male reproductive issues.