When frozen-thawed ram sperm is allowed to incubate at high concentrations, the rate of DNA damage is faster compared to lower concentrations. By investigating this, the study found that sperm DNA fragmentation changes over time not only based on the initial damage but also on sperm concentration during incubation. Lower sperm concentrations led to a slower increase in DNA damage. This discovery is crucial for techniques like artificial insemination, showing that managing sperm concentration post-thaw can affect DNA integrity. The research underscores the dynamic nature of sperm DNA fragmentation and highlights the need for consistent handling methods during reproduction procedures.