Six different types of papaya hybrids were tested to see which ones produced the most fruit and the highest amount of papain, an enzyme found in papayas. The hybrids CO-2 × Pusa Nanha and CO-5 × Pusa Nanha had the highest papain recovery, with CO-5 × Pusa Nanha showing the most enzyme activity. The hybrids CO-2 × Pusa Nanha, CO-4 × Pusa Nanha, CO-5 × Pusa Nanha, and CO-6 × Pusa Nanha were the best for fruit yield and earliness, and are recommended for further testing.