Exposing granulocytes to cryoprotectants like glycerol, dimethyl sulfoxide, and ethylene glycol can damage their cell membranes due to osmotic stress. The integrity of the membranes was affected by the type and concentration of cryoprotectants used. Glycerol was found to be the most damaging, followed by ethylene glycol and then dimethyl sulfoxide. Dimethyl sulfoxide was effective even at low concentrations. Higher temperatures also worsened membrane integrity. Adding sucrose or mannitol to ethylene glycol or dimethyl sulfoxide helped protect the cells from osmotic stress. A concentration of 200mM of sucrose or mannitol reduced membrane damage caused by dimethyl sulfoxide.