In this study, researchers looked at how sorting sperm for gender in red deer affects quality. They compared sorted and non-sorted sperm after thawing and found that non-sorted sperm are more motile and have less damaged DNA than sorted sperm. When exposed to oxidative stress, non-sorted sperm showed more DNA damage. However, sorted sperm had better resistance to oxidative stress. Adding a compound called GSH improved the motility of both sorted and non-sorted sperm under stress. This means the sperm sorting process can harm sperm quality, but sorted sperm are better at handling stress. GSH could help maintain quality in stressed sperm, unlike Trolox, which hinders sperm movement.