Grafting tobacco plants can increase key enzyme activities and chlorophyll content. When using specific rootstock and scion combinations, grafted tobacco seedlings showed higher nitrate reductase and glutamine synthetase activities, as well as more chlorophyll, compared to non-grafted seedlings. These differences were more pronounced when nitrogen levels were varied, with grafted plants maintaining higher enzyme activities and chlorophyll content as nitrogen decreased. This suggests that grafting can improve nitrogen efficiency in tobacco plants, leading to better growth and nutrient utilization.