This research looked at how human oviductal fluid and a special cell culture medium affect the acrosome reactions of human sperm. They compared these fluids to a regular cell culture fluid. Results showed that both the oviductal fluid and the special culture medium increased the rate of acrosome reactions in both fertile and infertile men's sperm after 3 hours. Moreover, after 6 hours, the special medium had a higher effect on acrosome reactions than the oviductal fluid. It was also noted that infertile men's sperm were more likely to die after an acrosome reaction compared to fertile men's sperm. In summary, the study found that these fluids can boost human sperm reactions, especially in infertile men, but the sperm might face a higher risk of dying after the reaction occurs.