Nitrogen fertilization affects the yield and dry matter of high-quality rice Tianyou 3301. The study found that the optimal nitrogen application for the highest yield was 205.06 kg·hm-2, with an economical application of 192.70 kg·hm-2. Using the right amount of nitrogen can increase spike size, spikelets, and seed set, leading to higher yields. Moderate nitrogen levels also boost dry matter production during growth periods. Crop growth rate is influenced by nitrogen application, with a linear correlation in the vegetative period and a parabolic correlation in the middle and late growth stages. To improve growth rates in these stages, heavy nitrogen fertilization is necessary.