The goal was to see how different freezing methods and chemicals affect the development of mouse embryos. They froze mouse embryos using four chemicals (PROH, EG, DMSO, G) with either a slow-freezing or a Vit-Master vitrification method. Results showed that using PROH with slow-freezing was best for embryo survival and growth. However, using EG with Vit-Master vitrification worked better than other chemicals for embryo viability and development. So, PROH is good for slow-freezing, while EG is better with Vit-Master vitrification, suggesting a new method to preserve mouse embryos effectively.