Inbred mouse embryos were successfully frozen using a method called vitrification at different temperatures. The embryos were treated with certain solutions before being plunged into liquid nitrogen for preservation. The embryos frozen at 25℃ had the best survival rates compared to those frozen at 20℃ or 28℃. After thawing, the survival rates of different types of embryos ranged from 77% to 90%. This study shows that vitrification at 25℃ is effective for preserving inbred mouse embryos, which is crucial for future research and applications.