The study investigated the characteristics of Morus multicaulis cv. Heyebai to help improve cultivation techniques. The biennial plants showed high efficiency in using weak light for photosynthesis. They also had a high photosynthetic potential and water utilization efficiency. Factors like stomatal conductance, vapor pressure, and light intensity were found to directly influence the plants' photosynthetic rate. The results suggest that planting this mulberry cultivar in dense formations with specific spacing can increase leaf yield. Additionally, growing the plants on slopes can help maximize sunlight and land use in hilly areas.