The study looked at how different amounts of nitrogen affected the growth and nitrogen levels in tobacco plants. They found that more nitrogen led to more aboveground growth, but the roots grew the most with a medium amount of nitrogen. The balance between roots and shoots changed as the plants grew. The plants with medium nitrogen had the most nitrogen overall, and it was distributed evenly in the plant. The way the nitrogen was absorbed by the plants also changed with the amount of nitrogen applied. Overall, using a moderate amount of nitrogen helped the plants grow better.