The study looked at how different methods of activating eggs, treating sperm, and using cysteine affected the development of pig embryos created through a specific type of fertilization. They found that using a combination of A23187 and 6-DMAP for egg activation led to better embryo development. Freezing and thawing sperm without cryoprotectant helped with the formation of male pronucleus in the embryos. Also, treating the embryos with cysteine for 4 hours after fertilization improved fertilization and development rates.