Cellulase enzymes sticking to lignin in corn stover can hinder the breakdown of cellulose into glucose, reducing biofuel production. Scientists studied how liquid hot water treatment affects this interaction. They found that the pretreatment changed the lignin's properties, making it more likely to bind to cellulase. Different types of cellulase were affected differently, with cellobiohydrolase being the most impacted. The way cellulase binds to lignin can affect how well it breaks down different types of biomass. The interaction is influenced by hydrophobic and electrostatic forces.