In this research, scientists studied boar sperm to understand changes in specific proteins during different reactions within the sperm. They wanted to see if these proteins could show whether a true or false acrosome reaction occurred. The researchers discovered that a protein called SPACA1 changed its location and size depending on the type of reaction happening in the sperm. When the acrosome reaction happened naturally, the SPACA1 protein was found in a specific region, but in damaged sperm, this protein was missing from there. Additionally, smaller versions of the SPACA1 protein increased in certain reactions. This specific protein pattern can be used to determine if the true acrosome reaction has taken place in boar sperm.