The study looked at how exposure to malathion affects the testicular enzyme activities and sperm quality of male rats. Male rats were given different doses of malathion for 60 days, and their testicular enzyme activities and sperm quality were measured. The results showed that malathion caused a decrease in testis and body weight, reduced sperm counts and motility, increased sperm malformation rate, and altered the activities of certain testicular enzymes. The highest dose of malathion had the most significant effects on the rats compared to the control group.