The study looked at how cooling boar semen in different extenders before freezing affects sperm quality. They tested semen from five boars in four extenders and held it at two temperatures for different times. Results showed that the extenders and holding time had a significant impact on sperm quality after freezing. Sperm motion patterns were different before freezing, with higher values in semen held at 10°C for 24 hours. Post-thaw sperm quality was better in certain extenders, with higher mitochondrial membrane potential and acrosome integrity. Holding semen at 10°C for 24 hours improved sperm quality after freezing, depending on the extender used.