Researchers found that using dilute acid to treat rice straw before enzymatic hydrolysis resulted in a high sugar yield of 62.59 g/100 g dry rice straw. The best conditions were 4% H2SO4 for 30 minutes, which extracted 80% of the total sugar. They also discovered that using simultaneous saccharification and fermentation (SSF) produced more ethanol and at a faster rate compared to separate hydrolysis and fermentation (SHF). In SSF, ethanol concentration reached 9.21 g/l with a productivity of 0.30 g/l h in 30 hours, while SHF only achieved 6.10 g/l and 0.16 g/l h in 36 hours. This study shows that dilute acid pretreatment can effectively produce biosugars from rice straw for ethanol fermentation.