Biochemistry, Genetics and Molecular Biology
8 years ago

Simple tweak boosts Aspergillus transformation rates, revolutionizing genetic engineering.

Fungal Genetics Reports
A simple modification of the A. nidulans transformation protocol increases the transformation frequency
J A Cockram, Sealy-Lewis

Paper Summary

Transforming Aspergillus nidulans with plasmid DNA can be more successful if the transformation plates are pre-incubated at room temperature for 24 hours before moving to 37C. This simple change has been shown to increase the frequency of successful transformations.

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