The researchers studied how different diluents and sperm concentrations affect the movement of horse sperm. They used a computerized system to analyze the sperm's speed and motion. The results showed that diluting the sperm to a concentration of 50 x 106 sperm/mL led to better sperm movement compared to lower concentrations. The diluent called BotuCrio® resulted in higher sperm motility and speed compared to other diluents tested. Overall, the study found that the choice of diluent and sperm concentration can significantly impact the quality of equine sperm movement.