Trametes versicolor was used to break down bisphenol A (BPA) in agricultural waste, while also producing laccase. Wheat bran and corn straw were good for laccase production. BPA degradation and laccase production happened together, with BPA boosting laccase production. Laccase activity peaked on days 6-10, degrading over 90% of BPA in 10 days without needing mediators. The best pH for BPA breakdown was 5.0, and some metal ions hindered degradation. Acetosyringone helped BPA breakdown the most. T. versicolor and laccase removed over 90% of BPA's estrogenic activity. This shows that T. versicolor and laccase can effectively treat BPA in industrial wastewater.