In the study, scientists looked at proteins in sperm from men with poor sperm movement (asthenozoospermia) and compared them to sperm from healthy men. They found 152 different proteins that were changed in asthenozoospermia, with 84 higher and 68 lower than normal. From these, they focused on an enzyme called GPI, crucial for sperm energy production. GPI was less in asthenozoospermic sperm, affecting their movement. By adding a related substance, fructose-6-phosphate, sperm movement was improved in the lab. This suggests that problems in GPI-related energy processes could be a reason for poor sperm movement in asthenozoospermia. The study provides new insights into how sperm movement works and how it could be enhanced in men with asthenozoospermia.