This research looked at how treating rye straw with weak sulfuric acid affects turning it into sugar for making ethanol. They tested different acid strengths and enzymes on the straw. They found that using a specific acid strength and enzymes in a certain buffer made the most sugar. By adding yeast to the sugary mix, they made the most ethanol. The best method from this study produced about half the maximum expected amount of ethanol from the straw. Using a mixture of yeasts made the most ethanol output. This shows that treating rye straw with acid and using specific enzymes can help make biofuel efficiently.