The study looked at how different types of fertilizer affect the growth and yield of spinach plants. They tested biological organic fertilizer and a mix of organic and chemical fertilizer on spinach. The results showed that using only organic fertilizer led to better spinach growth in terms of leaf temperature, width, root length, plant height, and weight. The best formula for spinach production was E5, which resulted in significantly taller plants and heavier yields compared to other formulas tested. This research provides important information on the best fertilizer for spinach growth and development.