The article discusses how ecological processes in freshwater environments operate at large scales like watersheds and continents, but experiments are usually done on smaller scales. Organisms in freshwater vary greatly in size, from tiny microorganisms to large creatures, yet they are all made of the same materials. Understanding how organisms change with size can help us understand ecology better. The researchers suggest the need for methods to scale up experiments to whole systems to better conserve and manage freshwater habitats. Landscape ecology, macroecology, and macrosystems are important for studying spatial relationships and broad patterns in organism biology at large scales.