Dog semen was frozen using different substances to improve the freezing process. Comparing glycerol and ethylene glycol, glycerol worked better at a 4% concentration. Keeping the frozen sperm for 60 minutes at 4 ℃ before using it was also found to be more favorable. Results showed that using glycerol at 4%, and balancing for 60 minutes at 4 ℃, led to a higher recovery rate for canine sperm freezing, with an average of 39.21% recovery rate. This combination was found to be quite reliable for preserving canine semen.