A spore laccase from Bacillus subtilis WD23 was studied for its ability to break down dyes. The laccase showed activity similar to that of laccases, breaking down specific compounds. The optimal conditions for the laccase were a pH of 6.8 and a temperature of 60°C. It remained stable over a wide pH range and had a long half-life at certain temperatures. The laccase was sensitive to some solvents and inhibitors, with methanol, EDTA, and dithiothreitol strongly inhibiting its activity. However, it was only slightly affected by NaN3 and xylene. The spore enzyme efficiently decolorized anthraquinone and azo dyes within 24 hours.