The scientists studied how ozonized saline injections affect pain and inflammation in rats with foot incisions. They tested different amounts of ozonized saline on rats and checked behaviors and blood markers for pain and inflammation. The results showed that rats given higher doses of ozonized saline had less pain after surgery. Additionally, the levels of certain markers for inflammation were lower in rats treated with ozonized saline compared to those not treated. This suggests that ozonized saline can help reduce pain in rats and that the amount given affects how well it works. The study indicates that ozonized saline may have pain-relieving properties and could be used in managing post-surgery pain in the future.