The researchers studied how adding glycerol affects the quality of cat sperm during freezing and thawing. They found that a shorter glycerol equilibration time helps preserve sperm quality better. After thawing, sperm motility was higher in samples with a 5-minute equilibration time compared to a 10-minute one. Sperm viability was also better maintained in the 5-minute group. The freeze-thaw process is crucial for preserving sperm quality, with shorter equilibration times being more effective.