Organic fertilizer can improve soil quality and tomato yield in greenhouse cultivation. By replacing some chemical fertilizer with organic fertilizer, soil bacteria and actinomycetes increased, leading to better soil health. Organic fertilizer also boosted soil enzyme activity, organic matter, and nutrient levels, enhancing tomato quality and yield. The study found that using 15-30% organic fertilizer instead of chemical fertilizer resulted in the highest tomato yield and quality, making it a preferred fertilization method for this planting pattern.