The study aimed to compare how different freezing speeds and cryoprotectant concentrations affect the preservation of mouse subcutaneous tissue. Three cryoprotectants—dimethyl sulfoxide, glycerinum, and 1,2‑propanediol—were tested. Slow freezing led to more fibroblast growth (a type of cell) compared to rapid freezing. Using 10-30% glycerinum during slow freezing resulted in the highest fibroblast growth. Rapid freezing with 20% DMSO or 10% glycerinum showed some fibroblast growth, but other cryoprotectants didn't lead to fibroblast growth. Overall, slow freezing with glycerinum was the most effective method for preserving subcutaneous tissue and promoting cell growth.