The researchers wanted to improve the freezing process of ram semen for artificial insemination. They tested different concentrations of glycerol and ethylene glycol at different temperatures to see how it affected the sperm's movement after thawing. They found that adding 5% glycerol at 5°C or 30°C had different effects on sperm motility. The study suggests that the type and amount of cryoprotectants used can impact the quality of frozen ram semen.