The study looked at how different crop cycles affect the genetic variability, heritability, and yield of sugarcane. Ten sugarcane genotypes were tested over three crop cycles: plant cane, first ratoon, and second ratoon. Results showed that stalk length varied between crop cycles, while stalk diameter and weight decreased in older crops. Stalk number increased in first and second ratoon crops. Cane yield fluctuated among crop cycles, with a decrease in the second ratoon. Sugar yield followed a similar trend to cane yield. The potential for improvement through selection was highest in plant cane. Overall, genetic variability and heritability decreased from plant cane to second ratoon crop for certain traits, while slightly increasing for others.