Elevated carbon dioxide during the day, not at night, helps mulberry plants grow better and produce higher quality leaves. The study looked at how different CO2 levels affected mulberry seedlings over 120 days. Daytime high CO2 increased plant growth and nutrient levels in the leaves, while nighttime high CO2 had less impact on growth but improved leaf quality. However, continuous high CO2 both day and night decreased leaf yield and nutrient content. To sustainably grow mulberry plants in the future, it's important to use mineral nutrient fertilizers, especially nitrogen, phosphorus, iron, magnesium, and zinc.