The researchers studied different types of rice seeds to see how their proteins differ. They used a method called SDS PAGE to analyze the proteins. They found that restorer lines had two protein bands in the glutelin α 3 area, while other lines had only one. They also observed a faster-moving protein band in the restorer lines, which they named α 4f. The presence of these protein bands in the seeds followed a specific pattern in the F2 generation, indicating a genetic inheritance. The researchers traced the origin of certain protein bands in one of the lines back to a specific rice variety. Additionally, they found that one type of protein was easier to extract from one line compared to another.