Biochemistry, Genetics and Molecular Biology
4 years ago

Revolutionary Gene Boosts Rice Transformation Efficiency by Over 2800%

分子植物：英文版
Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency
Ye, Zheng, Xiaowei Xiaowei, He, Yinghui, Ying, Jiang-Feng, Lü, Stanton, Gelvin, Huixia, Shou

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The researchers enhanced rice transformation efficiency by introducing a gene from Arabidopsis thaliana called AtHTA1 under the control of a maize ubiquitin promoter. Rice plants expressing AtHTA1 showed a 2844% increase in transformation efficiency compared to control plants. Additionally, co-infection of rice calli with a vector containing AtHTA1 and another vector carrying the target gene resulted in a 2750% increase in transformation. This means that introducing AtHTA1 temporarily or stably improved rice transformation efficiency.