The scientists tested different mixtures of protective substances to freeze and store jaguar cells for conservation. They looked at how the cells appeared, grew together, stayed alive, and functioned after being frozen and thawed. The cells frozen in ethylene glycol had lower survival rates, but those frozen in dimethyl sulfoxide with or without sucrose kept functioning well. This means a combination of dimethyl sulfoxide alone or with sucrose is good for freezing jaguar cells for future use. This research helps in protecting jaguars by preserving their cells effectively.