Sugar content in sugar beet roots is influenced by the activity of sucrose synthase (SS). A study looked at how different levels of nitrogen affected SS activity, root yield, and sugar content. They found that SS activity in roots was higher than in leaves. Increasing nitrogen levels led to higher SS decomposition activity and lower synthesis activity. SS activity was influenced by the ratio of nitrate to ammonium. Sugar content decreased with higher nitrogen levels but increased with more ammonium. The best results were seen with medium nitrogen levels and a nitrate to ammonium ratio of 1. This study helps farmers use nitrogen fertilizer more effectively for sugar beet production.