Researchers collected and studied semen from Magellanic penguins to understand how to preserve it for future use. They looked at how different temperatures and cryoprotectants affected the quality of the sperm. They found that both dimethylsulfoxide (DMSO) and ethylene glycol (EG) were effective in preserving the sperm quality during freezing and thawing. Sperm quality decreased over time, but samples maintained high numbers of viable and normal sperm. Clean ejaculates had better sperm quality compared to those with urate contamination. This study provides important information on penguin semen production and storage methods.