The scientists wanted to see how mouse sperm quality, fertility, and embryo development are affected by transporting sperm from the cauda epididymis at room temperature or 4-8 degrees Celsius with or without a special solution called M2. They found that fresh sperm had better quality and fertility compared to transported sperm. Sperm motility was similar to fresh sperm when stored at 4-8 degrees Celsius with M2. Transporting cauda epididymis at 4-8 degrees Celsius without M2 was best for maintaining sperm fertility and embryo development. However, transporting at room temperature without the solution could still produce embryos for live offspring.