The researchers studied how different solvents and gelatin amounts affect the properties of hydrogels used in 3D bioprinting. They found that using deionized water makes the hydrogels more rigid, while adding more gelatin increases their stiffness. Using a culture medium for bioink preparation is best for cell attachment and growth. The hydrogel with 2% alginate and 9% gelatin showed good printability and cell viability. The release of gelatin and calcium ions from the hydrogel affects cell survival.